Intracellular Fluorescent Probe Concentrations by Confocal Microscopy

Fluorescent ligands for studying neuropeptide receptors by confocal microscopy.

This paper reviews the use of confocal microscopy as it pertains to the identification of G-protein coupled receptors and the study of their dynamic properties in cell cultures and in mammalian brain following their tagging with specific fluorescent ligands. Principles that should guide the choice of suitable ligands and fluorophores are discussed. Examples are provided from the work carried ou...

Cytochemical studies with African Horse-Sickness Virus by Fluorescent Microscopy

Deep tissue fluorescent imaging in scattering specimens using confocal microscopy.

In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or...

Confocal microscopy of intracellular calcium dynamics during fertilization.

During fertilization in all animals investigated, fully developed eggs or meiotically immature oocytes must generate a proper change in their intracellular free calcium concentrations ([Ca]i) to develop normally (31). The first images of a fertilization-induced calcium response were obtained in fish oocytes that had been microinjected with the calcium-sensitive photoprotein, aequorin (4). Since...

Variations of the Normal Human Limbal Stem Cells Detected by In Vivo Confocal Microscopy

Background  To report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. Methods: This was a retrospective study of fourteen eyes from 11 healthy individuals. Confocal imaging of cornea and limbus was performed using Heidelberg Retina Tomograph III Rostock Corneal Module. Results: The typical structure of the palisades of Vogt (POV) was detected ...